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qPCR is relatively easy to perform as compared to movement cytometry that needs considerable know-how for reproducible success. Actually, stream cytometric enumeration of microbial cells was initially limited to pure cultures [31] and nonetheless continues to be complicated when done in advanced matrices [32].

our simulation details along with the Beforehand published scientific tests. Whilst relative taxon abundance may be indicative, complete quantification is needed for obtaining an extensive understanding of the dynamics and interactions in the microbiome.

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Using relative abundance from next generation sequencing (NGS) data can result in misinterpretations of microbial community structures as as a result of compositionality, the relative abundances on the taxa being mutually dependent. Therefore a boost of one taxon inevitably causes the concurrent decrease of another(s). Considering that the improvements of elements are mutually dependent, significant Bogus discovery premiums come about when compositional information are analyzed working with common statistical procedures [one].

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